Use
sub("_(?=[^_]*_[^_]*$)", "-", a, perl=TRUE)
See regex proof.
Explanation
--------------------------------------------------------------------------------
_ '_'
--------------------------------------------------------------------------------
(?= look ahead to see if there is:
--------------------------------------------------------------------------------
[^_]* any character except: '_' (0 or more
times (matching the most amount
possible))
--------------------------------------------------------------------------------
_ '_'
--------------------------------------------------------------------------------
[^_]* any character except: '_' (0 or more
times (matching the most amount
possible))
--------------------------------------------------------------------------------
$ before an optional
, and the end of
the string
--------------------------------------------------------------------------------
) end of look-ahead
See R proof:
a <- c("2018-01-09_B2_HILIC_POS_123_-14b_090.mzML", "2018-01-09_B2_HILIC_POS_243_-12a_026.mzML", "2020-01-09_B2_HILIC_POS_415_893a_059.mzML", "2020-01-18_B3_HILIC_POS_LV7001248356_040.mzML")
sub("_(?=[^_]*_[^_]*$)", "-", a, perl=TRUE)
Results:
[1] "2018-01-09_B2_HILIC_POS_123--14b_090.mzML"
[2] "2018-01-09_B2_HILIC_POS_243--12a_026.mzML"
[3] "2020-01-09_B2_HILIC_POS_415-893a_059.mzML"
[4] "2020-01-18_B3_HILIC_POS-LV7001248356_040.mzML"
与恶龙缠斗过久,自身亦成为恶龙;凝视深渊过久,深渊将回以凝视…