It should close the file, yes, though when exactly it does so is implementation-dependent. The reason is that there is no reference to the open file after the end of the list comprehension, so it will be garbage collected, and that will close the file.
In CPython (the regular interpreter version from python.org), it will happen immediately, since its garbage collector works by reference counting. In another interpreter, like Jython or Iron Python, there may be a delay.
If you want to be sure your file gets closed, it's much better to use a with
statement:
with open("file.txt") as file:
lines = [line.strip() for line in file]
When the with
ends, the file will be closed. This is true even if an exception is raised inside of it.
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