For the BIOS to load your boot sector from CD, you'll need to make the CD bootable by using the "El Torito" standard.
Once you use that, you have two options
a. Emulation - the BIOS emulates either a floppy or hard drive, and you can read your kernel through the INT13 calls with either device 00 or device 80.
b. The device doesn't emulate, and you can read directly from the CD using the INT13 ExtendedRead function.
To see how this is done, look at the Linux "ISOLINUX" loader - ISOLINUX.ASM
To provide a more specific starting point to your question, El Torito spec,section 5.3:
Once the system jumps to segment:0, the program can retrieve its boot
information by issuing INT 13, Function 4B, AL=01. After the boot process
has been initiated the INT 13 Extensions (functions 41-48) will access the
CD using 800 byte sectors and the LBA address provided to INT 13 is an
absolute sector number. This gives any program running in no emulation mode
the ability to locate the boot catalog, and any other information on the
CD, without providing a device driver.
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